Analysis Note

ControlU20S cell lysate

Application

Anti-Transgelin/SM22a Antibody, clone 20F2.2 is a highly specific mouse monoclonal antibody, that targets Transgelin & has been tested in western blotting & ICC.

Western Blotting Analysis: 1 µg/ml from a representative lot detected Transgelin/SM22a in 10 µg of NIH/3T3 and PC12 cell lysates.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Transgelin/SM22a in NIH/3T3 cells.

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Transgelin, also known as 22 kDa actin-binding protein, Protein WS3-10, and Smooth muscle protein 22-alpha (SM22-alpha), is an actin cross-linking protein that is involved in cellular calcium interactions and contractile properties of the cytoskeleton contributing to replication and senescence in smooth muscle tissue. Transgelin is overexpressed in senescent human fibroblasts.

Immunogen

GST-tagged recombinant protein corresponding to human Transgelin/SM22a.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in U20S cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Transgelin/SM22a in 10 µg of U20S cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~22 kDa observed